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描述
The TrackIt™ 100 bp DNA Ladder is suitable for sizing double-stranded DNA fragments from 100–1,500 bp. The ladder is prepared from a plasmid containing repeats of a 100 bp DNA fragment. The TrackIt™ 100 bp DNA Ladder is formulated with two unique tracking dyes, Xylene Cyanol FF (XCFF) and tartrazine, that allow you to visually track DNA migration during electrophoresis and also indicate when maximum resolution is achieved. The tracking dyes do not obscure the visualization of DNA bands in the ladder as the dyes run outside the limits of most DNA bands in the ladder. Important features of this ladder:
• Consists of 15 fragments in 100 bp increments ranging in size from 100–1,500 bp and an additional band at 2,072 bp
• 600 bp reference band is ~2-fold brighter than other bands for easy determination of band size
• Formulated with unique tracking dyes, XCFF and tartrazine
• Designed for use with E-Gel® agarose gels and TBE/TAE agarose gels
• Supplied in a ready-to-load format, stable at room temperature
• Visualized with ethidium bromide or SYBR® Green stainingFigures
The 100 bp DNA Ladder.
1.5% agarose gel was stained with ethidium bromide; 3 µg was loaded.
规格
Gel Compatibility: Agarose Gels, E-Gel® Ready to Load: Yes Size Range: 0.1 to 1.5 kb Product Size: 100 applications Concentration: 0.1 µg⁄µl Number of Reactions: 100 Applications Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
内容及储存
Contains: TrackIt™ 100 bp DNA Ladder (0.1 µg/µl) in 10 mM Tris-HCl (pH 7.5), 10 mM EDTA (pH 8.0), 0.06% XCFF, 0.6% tartrazine, and 5% glycerol.
Store at room temperature.化学品安全技术说明书(MSDS)
常见问题解答
What are linkage disequilibrium units (LDUs) the SNPbrowser™ Software?Can I use the 100 bp DNA ladder for quantitation on agarose gels?I’m seeing anomalous migration of my DNA ladder. What happened?
引用及参考文献
Santi S, Grisan S, Pierasco A, DE Marco F, Musetti R
Plant Cell Environ 2013; (36):2 343-355Carter L, Lindsey LA, Grim CJ, Sathyamoorthy V, Jarvis KG, Gopinath G, Lee C, Sadowski JA, Trach L, Pava-Ripoll M, McCardell BA, Tall BD, Hu L
Appl Environ Microbiol 2013; (79):2 734-737Mei Teh B, Redmond SL, Shen Y, Atlas MD, Marano RJ, Dilley RJ
Exp Cell Res 2013; (319):6 790-799
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