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描述
The 50 bp DNA Ladder is suitable for sizing double-stranded DNA from 50 to 800 bp. Prepared from a plasmid containing repeats of a 50 bp DNA fragment, the ladder consists of 16 blunt-ended fragments ranging in length from 50 to 800 bp, at 50 bp increments, and vector fragments >2,000 bp.
Product use
The double-stranded ladder can be visualized on 2% to 3% agarose gels after ethidium bromide staining. For easy reference on agarose gels, the 350 bp band is two to three times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase or T4 DNA polymerase.
Figures
The 50 bp DNA Ladder.
2% agarose gel stained with ethidium bromide; 0.5 µg/lane.
规格
Gel Compatibility: Agarose Gels Ready to Load: No Mass: 50 µg Size Range: 50 to 800 bp Product Size: 50 µg Concentration: 1 µg⁄µl Green Features: Less waste, Sustainable packaging Shipping Condition: Approved for shipment at Room Temperature or on Dry Ice
内容及储存
Contains: 50 bp DNA Ladder (1 µg/µL) in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA.
Store at -20°C.手册和实验方案
化学品安全技术说明书(MSDS)
常见问题解答
I’m seeing anomalous migration of my DNA ladder. What happened?What are linkage disequilibrium units (LDUs) the SNPbrowser™ Software?引用及参考文献
Kho EY, Wang HK, Banerjee NS, Broker TR, Chow LT,
Proc Natl Acad Sci U S A (2013) 110:7542-7549Romero-Quintana JG, Frías-Castro LO, Arámbula-Meraz E, Aguilar-Medina M, Dueñas-Arias JE, Melchor-Soto JD, Romero-Navarro JG, Ramos-Payán R
BMC Med Genet 2013; (14): 7-7Jittawuttipoka T, Planchon M, Spalla O, Benzerara K, Guyot F, Cassier-Chauvat C, Chauvat F
PLoS One 2013; (8):2 e55564-e55564
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