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描述
The 100 bp DNA Ladder is suitable for sizing double-stranded DNA from 100 to 1,500 bp. Prepared from a plasmid containing repeats of a 100 bp DNA fragment, the ladder consists of 15 blunt-ended fragments ranging in length from 100 to 1,500 bp, at 100 bp increments, and an additional fragment at 2,072 bp.
Product use
The double-stranded ladder can be visualized on 1% to 2% agarose gels after ethidium bromide staining. For easy reference on agarose gels, the 600 bp band is two to three times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.
Figures
The 100 bp DNA Ladder.
1.5% agarose gel was stained with ethidium bromide; 3 µg was loaded.
规格
Gel Compatibility: Agarose Gels Ready to Load: No Mass: 50 µg Size Range: 0.1 to 1.5 kb Product Size: 50 µg Concentration: 1 µg⁄µl Volume (Metric): 50 µl Green Features: Less waste, Sustainable packaging Shipping Condition: Approved for shipment at Room Temperature or on Dry Ice
内容及储存
100 bp DNA Ladder is supplied at 1 µg/µl in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA. Store at -20°C.手册和实验方案
化学品安全技术说明书(MSDS)
常见问题解答
Can I use the 100 bp DNA ladder for quantitation on agarose gels?How can I label DNA ladders?I’m seeing anomalous migration of my DNA ladder. What happened?
引用及参考文献
Pai Rama; Soreghan Brian; Szabo Imre L; Pavelka Meredith; Baatar Dolgor; Tarnawski Andrzej S;
Nat Med (2002) 8:289-382
Product usage: The Invitrogen 100 bp ladder was used to with agarose gel electrophoresis in a 1.25% agarose gel and was visualized with ethidium bromide staining.
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